跟随我们:
奉献/服务
提供50000每年向全球科研单位提供的服vwin电子竞技务和产品

单细胞分离的微发泡技术

感谢我们知识渊博的科学家和先进的仪器系统,,vwin使客户能够通过支持Native™抗体发现(单B细胞抗体发现)的微加工技术简化革命性抗体发现的工作流程。徳赢The single B cell antibody strategies harbor the potential to isolate native mAbs with the preservation of the natural VH and VL pairing that are present predominantly体内and difficult to emulatein vitro.Microengraving antibody 徳赢discovery system is specifically designed to provide rapid screening and detection of single cells secreting antibodies with the desired reactivity profiles from large populations of primary B cells for our clients with significant reductions in operational costs,时间和资源利用。

微发泡技术是如何工作的??

Based on the same principles as ELISA,microengraving technique can be applied as an extraordinary approach for single B cell antibody 徳赢discovery,它使用一种由软石版印刷方法制造的工程微型工具。Combined with the use of multiple donor species and immunization regimens,这种方法使我们能够产生大量多样的稳定的剧目,包括稀有抗体在内的高亲和力单克隆抗体。These high-quality antibody repertoires form the basis for a comprehensive high throughput screening effort to identify optimal antibody leads against targets of interest.微创技术利用亚纳米级井的微制备阵列,在空间上分离单个抗原特异性抗体分泌细胞(ASCS),并打印蛋白质微阵列。阵列上的每个点都含有由单个细胞分泌的抗原特异性IgG。微井阵列的超高通量筛选有助于发现独特的克隆变异。此外,使用微井阵列和适当格式的产量数据进行分析(区块,行,and column locations for wells of interest) significantly enhance the rate and success of recovery in either semi-automated or automated modes.

单细胞分离的微发泡技术图1微发泡技术示意图。

微发泡技术产生抗体的工作流程

微凝乳抗体产生技术使一个快速和高通量的系统用于筛选ASC,and subsequent recovery and clonal expansion of single B cells of interest.Microengraving technique was applied to detect and isolate auto-reactive Abs from primary B cells and collect multiparametric datasets that describe the specificity,许多分离的原代B细胞分泌的abs的同种型和表观亲和力。此外,已经产生了高通量方法,从而有效地鉴定具有所需特异性的B细胞。Isolated antigen-specific B cells will also provide nature VH and VL pairs,用RT-PCR和套式PCR进行扩增,用于深部测序或in vitro重组单克隆抗体生产。

单细胞分离的微发泡技术Fig.2 Workflow of antibody 徳赢discovery by microengraving system.

Significant Features of Microengraving Technology


vwin基于技术专长和技术诀窍,以最优质、最具竞争力的价格,通过微发泡技术提供一系列定制的单克隆抗体徳赢发现服务。Our excellent specialists exploit a highly automated and sophisticated process to effectively select,净化,生成并评估抗体,以促进您的研究或项目。如果您对我们的服务有任何要求或问题,please do not hesitate tocontact us了解更多详细信息。




相关章节

Services:

我们的客户服务代表每天24小时待命,from Monday to Sunday.Contact Us

网上查询
姓名:
*Phone:
*电子邮件地址:
*感兴趣的服务和产品:vwin电子竞技
Project Description:
*Verification Code:
Please input“比奥拉布“(不区分大小写)作为验证码。
Contact Us

USA
Tel: 1-631-381-2994
传真:1-631-207-8356
Email:

欧洲
电话:44-207-097-1828


Platforms

vwin电子竞技 膜蛋白平台 Hyperdoma™平台 酵母展示平台
?2007-2019创意生物实验室版权所有