跟随我们:
奉献/服务
To provide50000每年向全球科研单位提供的服vwin电子竞技务和产品

杂交瘤测序服务

在抗体开发领域有多年的经验,vwin建立了一个高精度的测序平台,为杂交瘤细胞株提供高质量的抗体序列测定服务。vwinCreative Biolabs' hybridoma sequencing service is highly versatile and is applicable for both IgG and IgM types of monoclonal antibodies produced by mouse and rat hybridoma cell lines.我们致力于提供一流的定制服务,为您的项目的成功作出贡献。

抗体序列的价值

杂交瘤测序服务

抗体的蛋白质序列是其最重要的属性之一,能够使各种下游应用和发展:

  1. 专利申请:抗体氨基酸序列的唯一性,especially that from the variable region,is the key factor that dictates the novelty of the antibody and plays a crucial role in the patent application to ensure the exclusive rights in downstream antibody developments.
  2. 抗体重组表达:抗体序列作为载体构建和重组表达的蓝图。
  3. 抗体下游开发:原抗体序列为各种下游工程平台提供了模板,如人性化和亲和力成熟。
  4. 其他应用:抗体序列也可用于杂交瘤细胞系的验证。antibody format alteration,and to serve as a precaution in case of the loss of hybridoma cell line due to contamination.

杂交瘤测序

杂交瘤测序是指获得编码杂交瘤细胞系产生的抗体的可变重(VH)和可变轻(VL)域的cDNA的序列信息的过程。在排序之前,total mRNA of the hybridoma cells is extracted followed by PCR amplification of antibody variable regions (VH and VL) as well as non-variable flanking constant region sequences.抗体序列信息由测序仪从扩增的PCR产物中获得。vwin电子竞技With antibody variable region protein sequence,通过将可变区域嫁接到不同的框架序列模板上,可以设计不同的抗体形式。Antibodies generated by this method can be produced通过哺乳动物表达系统中的重组表达。或者,全长序列也可以从杂交瘤测序中获得,以实现抗体的产生。通过recombinant expression and to serve as an insurance policy to secure the source of the antibody in case of losing the hybridoma cell line due to contamination or other reasons.

Schematic representation of the approach for high-throughput sequencing of the Ig sequence repertoire.图1。Schematic representation of the approach for high-throughput sequencing of the Ig sequence repertoire.(Georgiou,G.,2014)

Hybridoma sequencing service at vwinCreative Biolabs:

In this service,采用5'race方法进行放大,clone and sequence the complete sequences of both heavy and light chains of an antibody,which are subsequently cloned into two cloning vectors for downstream sequence manipulation and expression vector constructions.

In this service,只有在抗体可变区的重链和轻链的序列被专门设计的退化引物扩增并测序。The resulted variable region can be grafted into any framework to yield an Ig isoform that is most suitable for the client's project.

抗体序列,创新生物实验室的科学家提供将vwin序列转化为各种形式抗体的服务,including multi-isoform full-length antibodies and fragment antibodies such as Fab or scFv.一份详细的项目报告,以及包含抗体结构的载体,在项目结束时交付给客户。

服务特点


vwinCreative Biolabs also offershigh-throughput hybridoma platform以及其他与杂交瘤相关的服务,以促进您的抗体发现项目。徳赢Please feel free tocontact usfor more information.

Reference
1.乔治乌G.,(2014)。“抗体库高通量测序的前景和挑战。”自然生物技术,32(2),158-168.




Related Sections

Services:

我们的客户服务代表每天24小时待命,from Monday to Sunday.Contact Us

网上查询
姓名:
*Phone:
*电子邮件地址:
*感兴趣的服务和产品:vwin电子竞技
Project Description:
*Verification Code:
Please input“生物学实验室”(不区分大小写)作为验证码。
Contact Us

USA
Tel: 1-631-381-2994
传真:1-631-207-8356
Email:

欧洲
电话:44-207-097-1828


Platforms

vwin电子竞技 膜蛋白平台 Hyperdoma™平台 酵母展示平台
?2007-2019创意生物实验室版权所有