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Internalization Antibody Screening

创意生物实验室now offers high-throughput screening service of internalizing antibodies to our customers around the world.Integrated with multiple leading-edge technology platforms,we can guarantee our clients with the most efficient and reliable service to isolate antibody candidates with internalization potential against various membrane targets.

Receptor-mediated internalization is a critical characteristic of therapeutic antibodies.Upon binding membrane target,抗体受体复合物可以通过内吞作用主动转移到胞质溶胶中,导致降解或循环(主要是FCRN介导)。Fc介导的抗体内化与抗体密切相关。体内half-life;更重要的是,FV介导的抗体内化,as in most cases,能引起靶受体对细胞膜的下调,thus eliciting diverse downstream therapeutic effects.Many of the most famous antibody drugs on the market have internalization potentials as one important mechanism of action.

此外,具有内化功能的抗体可作为靶向药物输送的优良载体,toxins,enzymes or DNA into cells for many therapeutic/ diagnostic applications.In contrast to receptor-independent reagents that can penetrate cell membranes,receptor-mediated internalizing antibodies are able to confer cell-specific drug delivery,which is the fundamental requirement for targeted therapy,即平均每日住院人数。Notably,for this type of application,内部化能力有时取决于货物。允许一种货物内化的内化抗体/肽可能不支持不同货物的内化。Therefore,custom cargo-fusion antibody libraries are very necessary.

Internalizing Antibody Screening

拥有最先进的技术平台和抗体开发领域的丰富专业知识,vwin创新生物实验室提供了多种选择,以追求对给定表面受体的内化抗体:

Phage Display Library Internalization Screening
我们已经做了大量的全细胞噬菌体展示库筛选项目,以选择对各种细胞表面受体(如egfr)特异的内化抗体和肽。NTRk2,CTLA4型,HER2,CD20,CD3,CD59,转铁蛋白受体,and CD33.Our staff scientists also have extensive experience in the 徳赢discovery of novel marker receptors by selecting internalizing antibodies and peptides that are specific for certain cell types.开发的方法是减少不针对特定受体的噬菌体颗粒的内化数量,以排除仅与细胞表面结合的噬菌体颗粒,并选择性地识别能够交联受体而不仅仅结合的噬菌体颗粒。

Fluorogen Activating Protein
这项创新技术采用了一种新的报告标签,称为荧光原激活蛋白(FAP),对所指示的膜受体进行基因标记。与特定的可溶氟化合物结合后能显示荧光。我们先后应用了两种具有不同结合能力和细胞通透性的氟化物。In this way,细胞内和细胞外标签可以显示不同的颜色。Our simple,robust FAP-tag makes a powerful approach for high-throughput,抗体/受体内化和贩运的动态研究。

GPCR Internalization Stable Cell Line
vwinCreative Biolabs is professional in developingGPCR stable cell lines适用于各种应用,例如receptor function screening,亲和力测量,and internalization screening.特别地,通过过度表达表明gpcr与荧光蛋白融合,这些工程细胞株可以分析或分析化合物诱导受体内化的能力。Single cells with strong intracellular fluorescence will be selected via FACS.目前,there are over 100 GPCR internalization cell lines available in vwinCreative Biolabs.New strains can also be constructed upon request.

Scientists invwin专门根据客户的特殊需求和期望定制最合适的计划。Please contact us for more information.




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